細胞株の詳細データ
TKG 0779:: HT45
ID | TKG 0779 |
Cell name | HT45 |
Data accepted | February, 2019. |
Animal | Mouse x Mouse |
Scientific name | |
Sex | |
Case history | |
Tissue | B cell hybridoma |
Genetics | |
Life span | |
Morphology | Lymphoblast-like |
Medium | RPMI1640 + 10% FBS |
Passage method | Dilution. |
Characteristics | TLR4-/- mice on C57BL/6 genetic background were immunized with Ba/F3-transfected
cells expressing human TLR4/MD-2. Mouse - Mouse hybridoma producing mouse
monoclonal antibody, IgG1, kappa. This mAb shows reactivity against human
TLR4 irrespective of the presence of MD-2. No cross-reactivity with mouse
TLR4. HAT resistance. This cell line can be used for immunoprecipitation
and flow cytometry. Transplantation into C.B-17/Icr-scid/scid mice induces ascites. |
Temperature | 37℃ |
CO2 concentration | 5% |
Subculture frequency | 1:1 ~ 1:2, twice a week. |
Classification | |
Mycoplasma | PCR: -, Hoechst DNA staining: - |
Established by | Tsukamoto, H. |
References | Int Immunol. 2012 24(8):495-506(PMID: 22499954). Biochem Biophys Res Commun. 2013 440(1):31-6(PMID: 24021278). FEBS Lett. 2015 589(24 Pt B):3893-8(PMID: 26602081). FEBS Lett. 2017 591(16):2406-2416(PMID: 28741733). |
References by users | |
Comments | |
Deposited by | Tsukamoto, H. |
Restriction | C: A prior written permission of the originator is necessary. |
Photo |