ID	TKG 0780
Cell name	HT53
Data accepted	February, 2019.
Animal	Mouse x Mouse
Scientific name
Sex
Case history
Tissue	B cell hybridoma
Genetics
Life span
Morphology	Lymphoblast-like
Medium	RPMI1640 + 10% FBS
Passage method	Dilution.
Characteristics	TLR4-/- mice on C57BL/6 genetic background were immunized with Ba/F3-transfected cells expressing human TLR4/MD-2. Mouse - Mouse hybridoma producing mouse monoclonal antibody, IgG1, kappa. This mAb shows reactivity against human TLR4 irrespective of the presence of MD-2. No cross-reactivity with mouse TLR4. HAT resistance. This cell line can be used for immunoprecipitation and flow cytometry. Transplantation into C.B-17/Icr-scid/scid mice induces ascites.
Temperature	37℃
CO2 concentration	5%
Subculture frequency	1:1 ~ 1:4, twice a week.
Classification
Mycoplasma	PCR: -, Hoechst DNA staining: -
Established by	Tsukamoto, H.
References	Int Immunol. 2012 24(8):495-506(PMID: 22499954). Biochem Biophys Res Commun. 2013 440(1):31-6(PMID: 24021278). FEBS Lett. 2015 589(24 Pt B):3893-8(PMID: 26602081). FEBS Lett. 2017 591(16):2406-2416(PMID: 28741733).
References by users
Comments
Deposited by	Tsukamoto, H.
Restriction	C: A prior written permission of the originator is necessary.
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