細胞株の詳細データ
TKG 0787:: HTB1
ID | TKG 0787 |
Cell name | HTB1 |
Data accepted | February, 2019. |
Animal | Mouse x Mouse |
Scientific name | |
Sex | |
Case history | |
Tissue | B cell hybridoma |
Genetics | |
Life span | |
Morphology | Lymphoblast-like |
Medium | RPMI1640 + 10% FBS |
Passage method | Dilution. |
Characteristics | TLR4-/- mice on C57BL/6 genetic background were immunized with Ba/F3-transfected
cells expressing human TLR4/MD-2. Mouse - Mouse hybridoma producing mouse
monoclonal antibody, IgG2a, kappa. This mAb shows reactivity against human
TLR4 irrespective of the presence of MD-2. No cross-reactivity with mouse
TLR4. HAT resistance. This cell line can be used for immunoprecipitation
and flow cytometry. Transplantation into C.B-17/Icr-scid/scid mice induces ascites. |
Temperature | 37℃ |
CO2 concentration | 5% |
Subculture frequency | 1:1 ~ 1:4, twice a week. |
Classification | |
Mycoplasma | PCR: -, Hoechst DNA staining: - |
Established by | Tsukamoto, H. |
References | FEBS Lett. 2015 589(24 Pt B):3893-8(PMID: 26602081). |
References by users | |
Comments | |
Deposited by | Tsukamoto, H. |
Restriction | C: A prior written permission of the originator is necessary. |
Photo |