細胞株の詳細データ

TKG 0789:: hLp4

ID TKG 0789
Cell name hLp4
Data accepted February, 2019.
Animal Mouse x Rat
Scientific name
Sex
Case history
Tissue B cell hybridoma
Genetics
Life span
Morphology Lymphoblast-like
Medium RPMI1640 +10% FBS or Hybridoma SFM.
Passage method Dilution.
Characteristics Wistar rats were immunized with human LBP N-terminal domain (aa1-212) recombinant protein named human lipopolysaccharide (LPS) - binding protein (LBP). Mouse - Rat hybridoma producing rat monoclonal antibody, IgM, kappa.
HAT resistance. This cell line can be used for ELISA and western blotting. This inhibits LBP-LPS binding.
Temperature 37℃
CO2 concentration 5%
Subculture frequency 1:1 ~ 1:4, twice a week.
Classification
Mycoplasma PCR: -, Hoechst DNA staining: -
Established by Tsukamoto, H.
References
References by users
Comments
Deposited by Tsukamoto, H.
Restriction C: A prior written permission of the originator is necessary.
Photo