細胞株の詳細データ
TKG 0789:: hLp4
ID | TKG 0789 |
Cell name | hLp4 |
Data accepted | February, 2019. |
Animal | Mouse x Rat |
Scientific name | |
Sex | |
Case history | |
Tissue | B cell hybridoma |
Genetics | |
Life span | |
Morphology | Lymphoblast-like |
Medium | RPMI1640 +10% FBS or Hybridoma SFM. |
Passage method | Dilution. |
Characteristics | Wistar rats were immunized with human LBP N-terminal domain (aa1-212) recombinant
protein named human lipopolysaccharide (LPS) - binding protein (LBP). Mouse
- Rat hybridoma producing rat monoclonal antibody, IgM, kappa. HAT resistance. This cell line can be used for ELISA and western blotting. This inhibits LBP-LPS binding. |
Temperature | 37℃ |
CO2 concentration | 5% |
Subculture frequency | 1:1 ~ 1:4, twice a week. |
Classification | |
Mycoplasma | PCR: -, Hoechst DNA staining: - |
Established by | Tsukamoto, H. |
References | |
References by users | |
Comments | |
Deposited by | Tsukamoto, H. |
Restriction | C: A prior written permission of the originator is necessary. |
Photo |