ID |
TKG 0791 |
Cell name |
M14-10 |
Data accepted |
February, 2019. |
Animal |
Mouse x Rat
|
Scientific name |
|
Sex |
|
Case history |
|
Tissue |
B cell hybridoma |
Genetics |
|
Life span |
|
Morphology |
Lymphoblast-like |
Medium |
RPMI1640 + 10% FBS |
Passage method |
Dilution. |
Characteristics |
Wistar rats were immunized with mouse CD14-expressing NRK-52E transfected
cells. Mouse - Rat hybridoma producing rat monoclonal antibody, IgG2b,
kappa. HAT resistance. This cell line can be used for immunoprecipitation
and flow cytometry. No cross-reactivity with human CD14. Transplantation
into C.B-17/Icr-scid/scid mice induces ascites. |
Temperature |
37℃ |
CO2 concentration |
5% |
Subculture frequency |
1:1 ~ 1:9, twice a week. |
Classification |
|
Mycoplasma |
PCR: -, Hoechst DNA staining: - |
Established by |
Tsukamoto, H. |
References |
Int Immunol. 2010 22(4):271-80(PMID: 20133493). |
References by users |
|
Comments |
|
Deposited by |
Tsukamoto, H. |
Restriction |
C: A prior written permission of the originator is necessary. |
Photo |
|