細胞株の詳細データ
TKG 0796：： M14-21
| ID | TKG 0796 |
| Cell name | M14-21 |
| Data accepted | February, 2019. |
| Animal | Mouse x Rat |
| Scientific name | |
| Sex | |
| Case history | |
| Tissue | B cell hybridoma. |
| Genetics | |
| Life span | |
| Morphology | Lymphoblast-like |
| Medium | RPMI1640 + 10% FBS |
| Passage method | Dilution. |
| Characteristics | Wistar rats were immunized with mouse CD14-expressing NRK-52E transfected
cells. Mouse - Rat hybridoma producing rat monoclonal antibody, IgG2a.
HAT resistance. This cell line can be used for immunoprecipitation and
flow cytometry. It weakly inhibits LPS binding of CD14. No cross-reactivity with human CD14. Transplantation into C.B-17/Icr-scid/scid mice induces ascites. |
| Temperature | 37℃ |
| CO2 concentration | 5% |
| Subculture frequency | 1:4, twice a week. |
| Classification | |
| Mycoplasma | PCR: -, Hoechst DNA staining: - |
| Established by | Tsukamoto, H. |
| References | Int Immunol. 2010 22(4):271-80(PMID: 20133493). |
| References by users | |
| Comments | |
| Deposited by | Tsukamoto, H. |
| Restriction | C: A prior written permission of the originator is necessary. |
| Photo |  |