ID |
TKG 0815 |
Cell name |
PC12-P1F1 |
Data accepted |
August, 2024. |
Animal |
Rat. |
Scientific name |
Rattus norvegicus. |
Sex |
|
Case history |
|
Tissue |
Pheochromocytoma. |
Genetics |
|
Life span |
Infinite. |
Morphology |
|
Medium |
DMEM (high glucose) + 5% FBS + 5% HS (Gibco 16050-130). |
Passage method |
0.05% trypsin / 0.02% EDTA-PBS. |
Characteristics |
PC12-derived subclone. PC12-P1F1 is hypersensitive for neuritogenesis to
temperature-controlled repeated thermal stimulation (TRTS). BMP4- or TRTS-induced
neuritogenesis is increased in PC12-P1F1 cells compared to parental PC12
cells. |
Temperature |
37℃ |
CO2 concentration |
5% |
Subculture frequency |
1:3, twice a week. |
Classification |
|
Mycoplasma |
PCR: -, Hoechst DNA staining: - |
Established by |
Kudo T. (Division of Oral Physiology, Tohoku University Graduate School
of Dentistry.) |
References |
Int J Mol Sci. 2020 Nov 7;21(21):8356(PMID: 33171774). |
References by users |
Int J Mol Sci. 2022 Dec 9;23(24):15602(PMID: 36555248). |
Comments |
|
Deposited by |
Kudo T. (Division of Oral Physiology, Tohoku University Graduate School
of Dentistry.) |
Restriction |
|
Photo |
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