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Direction for use of Automator (for mouse adipose tissues)


Exposure conditions

13 weeks aged C57BL/6N mouse was treated by inhalation anesthesia. Prohibiting interference of X-ray, the animals laid on a polystyrene foam bed during exposure.

Latheta LCT 200 (Hitachi-Aloka Medical) was used in this research. Exposure setting is below, Field : 48mm, Number of pixels : 512, Size of pixels : 96, Thickness of slices : 192m, Interspace of slices : 1080m, Cumulated number : 2, with respiratory-gated imaging.


This software supports Mac OS 10.7 (Lion) and later.


Protocol

@ Download manual HERE!

A Download automator tools HERE.

B Install antomator tools.


Dr. Akihiro Nakaya also has a part of the copyright of this program. Please refer to Dr. Akihiro Nakaya ( Niigata Univ.) and Keiko Ogura (IDAC, Tohoku Univ.) in any presentation at meetings and an article with images or graphs that you made with this program. 



Direction for use of Automator (for creating ROI histogram)


1. introduction

ROI_histgram is an Automator (*1) to create histograms of CT images from input data of CT values.
You can make the ROI_histgramfs input data using a ROI select tool in  OsiriX.
In this manual, we explain the procedure from selecting ROI in OsiriX to creating the histogram of the ROIfs CT values.
(*1) Automator is an application developed by Apple Inc. for OS X.


2. Select and Save ROI in OsiriX

In this section, we explain the procedure below to select a ROI in OsiriX and to save its CT value information  as an XML file.

(i) Download matome2 folder HERE.

(ii) Make folder(*2) under the hmatome2h folder. We recommend to label the date on new folder (ex:2015_3_14).
(*2: The folder should be in the same folder with gROI_histgramh)

(iii) Boot OsiriX and display the slice, in which you want to select a ROI.
@

(iv) Select the ROIfs form by clicking the right mouse button (or menu   bar) on the
slice (Our recommend form is Oval).

(
D) Select the ROI by clicking the left mouse button long time on the slice.

(
E) Click ROI ROI info in the menu bar.

(
F) Set the name (*2) of the file and click gExport to XMLh in new window.(*2: We recommend that name is consists of only digit and alphabet. gSpaceh or other symbols will not be identified in this system.

(
G) Save the XML file to the folder which you made in Step.(i).


(H) Set the XML file name as gmouseID _sliceNo.h



3. Create the Histogram

Drug & Drop the folder which you made (the name is the date) to gROI_histgramh.  And you will get the file gmouseID_sliceNo_hist.csv.h in the folder. You will open the file in Office (Microsoft or OpenOffice, etc.).

Copyright@acknowledgement
Please refer to Keiko Ogura (IDAC., Tohoku Univ.) and Keisuke Izumi (GSIS., Tohoku Univ.) in any presentation at meetings and/or any article with images or graphs that you made with our program.
 







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