細胞株の詳細データ

TKG 0195 :: FLS 5

ID TKG 0195
Cell name FLS 5
Data accepted Aug., 1991.
Animal Mouse (C57BL/6J).
Scientific name Mus musculus.
Sex
Case history
Tissue Embryo liver (stromal cells).
Genetics
Life span Finite.
Morphology Epithelial-like.
Medium RITC80-7 + 2% FBS + insulin + transferrin + EGF.
Passage method 0.1% trypsin.
Characteristics The cell line supports the proliferation and differentiation of the erythroid progenitor cells from mouse fetal liver and bone marrow. Use of type I collagen-coated plate (6 well) is better for good proliferation.
Temperature 37℃
CO2 concentration 5%
Subculture frequency 1:1 ~ 1:3, twice a week.
Classification
Mycoplasma PCR: -, Hoechst DNA staining: ±
Isozyme analysis NP
Established by Yanai, N. (Cell Biology, Inst. Develop. Aging and Cancer, Tohoku Univ.)
References Development, 110, 379-384, 1990. PMID: 2133543
References by users
Comments
Deposited by
Restriction In publishing the research results obtained by use this cell line, a citation of the following literature is necessary.
Development, 110, 379-384, 1990. PMID: 2133543
Photo