細胞株の詳細データ

TKG 0205 :: HepG2

ID TKG 0205
Cell name HepG2
Data accepted March, 1991.
Animal Human. (male, 15 y. o.).
Scientific name Homo sapiens.
Sex
Case history
Tissue Liver.
Genetics
Life span Infinite.
Morphology Epithelial-like.
Medium DMEM + 10% FBS (30% conditioned medium) or GIT.
Passage method 0.25% trypsin.
Characteristics Hepatoma (hepato cellular), α-fetoprotein(+), albumin(+). HLA-A 02/2402. A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210.
Temperature 37℃
CO2 concentration 5%
Subculture frequency 1:2 ~ 1:4, twice a week.
Classification
Mycoplasma PCR: -, Hoechst DNA staining: -
Established by
References Nature, 282, 615-616, 1979(PMID: 233137)., Science, 209, 497-499, 1980(PMID: 6248960)., In Vitro. Cell. Dev. Biol., 23, 349-354, 1987(PMID: 3034851).
References by users Biochem. Biophys. Res. Commun., 249, 131-137, 1998(PMID: 9705844)., Biochem. Biopys. Res. Commun., , 270, 649-656, 2000(PMID: 10753678)., Int. J Oncology, 18, 1175-1179, 2001(PMID: 11351248)., Int. J. Mol. Med., 10, 287-291, 2002(PMID: 12165802)., Oncol. Rep., 9, 1233-1236, 2002(PMID: 12375026)., Hepatology, 37, 1086-1096, 2003(PMID: 12717389)., Oncol. Rep., 10, 415-420, 2003(PMID: 12579282)., Oncol. Rep., 10, 671-677, 2003(PMID: 12684642)., Int. Immunol., 16, 777-786, 2004(PMID: 15096476)., J. Biol. Chem., 282, 23532-23540, 2007(PMID: 17573348)., Biochem. Biophys. Res. Commun., 366, 110-116, 2008(PMID: 18053801)., J. Control. Release, 136, 213-219, 2009(PMID: 19233236), Cancer Med. 2013 Jun;2(3):305-15(PMID: 23930207), Toxicol Lett. 2015 Jan 22;232(2):393-402(PMID: 25448278), Chem Pharm Bull. 2018;66(6):682-687(PMID: 29863070), Technol Cancer Res Treat. 2020 Jan-Dec:19:1533033820980077(PMID: 33334271).
Comments
Deposited by Sagami, I.(RITC, Tohoku Univ.).
Restriction
DNA Profile
(STR-PCR)
D5S818 D13S317 D7S820 D16S539 vWA TH01 Amelogenin TPOX CSF1PO
11,12 9,13 10 12,13 17 9 XY 8,9 10,11
Photo