| ID： |
TKG 0227 |
| Cell name： |
MIA Paca 2 |
| Data accepted： |
|
| Animal： |
Human (65 y. o. male Caucasion). |
| Scientific name： |
Homo sapiens. |
| Sex： |
|
| Case history： |
|
| Tissue： |
Pancreas. |
| Genetics： |
|
| Life span： |
Infinite. |
| Morphology： |
Epithelial-like. |
| Medium： |
DMEM + 10% FBS. |
| Passage method： |
0.25% trypsin. |
| Characteristics： |
Doubling time:40h, colony-forming efficiency 19% (soft agar), sensitive to
asparaginase. Pancreatic cancer.
|
| Temperature： |
37℃ |
| CO2 concentration： |
5% |
| Subculture frequency： |
1:4～1:10, twice a week. |
| Classification： |
|
| Mycoplasma： |
PCR: -, Hoechst DNA staining: - |
| Established by： |
Yunis, A. |
| References： |
Int. J. Cancer, 19, 128-135, 1977(PMID: 832918)., Cell. Mol. Life Sci., 53, 435-441, 1997(PMID: 9176562). |
| References by users： |
Biochem. Biophys. Res. Commun., 246, 205-209, 1998(PMID: 9600093)., J. Pathol., 196, 324-330, 2002(PMID: 11857496). |
| Comments： |
|
| Deposited by： |
|
| Restriction： |
|
DNA Profile
（STR-PCR) |
| D5S818 |
D13S317 |
D7S820 |
D16S539 |
vWA |
TH01 |
Amelogenin |
TPOX |
CSF1PO |
| 12, 13 |
12, 13 |
12, 13 |
10, 13 |
15 |
9, 10 |
X |
9 |
10 |
|
| Photo： |
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