細胞株の詳細データ

TKG 0232 :: NC37

ID TKG 0232
Cell name NC37
Data accepted
Animal Human.
Scientific name Homo sapiens.
Sex
Case history
Tissue Peripherel blood lymphocytes (B cell).
Genetics
Life span Infinite.
Morphology Lymphocyte-like.
Medium RPMI-1640 + 10% FBS or GIT.
Passage method Dilute 1/4 every 3-4 days.
Characteristics HGPRT(-), resistant to ouabain, partner cells for human B hybridoma. Ig nonproducer, human B lymphoblastoid cell line. Evidence from DNA fingerprinting indicates that this line and Raji (TKG0371) are derived from the same individual.
Temperature 37℃
CO2 concentration 5%
Subculture frequency
Classification
Mycoplasma PCR: -, Hoechst DNA staining: -
Isozyme analysis LD
Established by Uno, M. (Nihon Univ.).
References
References by users
Comments
Deposited by Uno, M.
Restriction
DNA Profile
(STR-PCR)
D5S818 D13S317 D7S820 D16S539 vWA TH01 Amelogenin TPOX CSF1PO
10, 13 13 10 8, 11 16, 19 6, 7 XY 8, 13 10, 12
Photo
 Photo(x100)