細胞株の詳細データ

TKG 0299 :: NIH/3T3

ID: TKG 0299
Cell name: NIH/3T3
Data accepted: April, 1992.
Animal: Mouse (Swiss).
Scientific name: Mus musculus
Sex:
Case history:
Tissue: Embryonal fibroblast.
Genetics:
Life span: Infinite.
Morphology: Fibroblast-like.
Medium: DMEM + 10% calf serum.
Passage method: 0.05% trypsin, 3 x 105 cells/6 cm dish, every 3 days.
Characteristics: Contact inhibition (+), useful for oncogene study.
Temperature: 37℃
CO2 concentration: 5%
Subculture frequency:
Classification:
Mycoplasma: PCR: -, Hoechst DNA staining: -
Isozyme analysis: LD
Established by: Todaro, G. J.
References: J. Cell Biol., 17, 299-313, 1963(PMID: 13985244)., J. Virol., 4, 549-553, 1969(PMID: 4311790)., Oncologia (Jap.), 17, 47-57, 1986.
References by users: Bioorg. Med. Chem. Lett., 14, 2563-2566, 2004(PMID: 15109652).
Comments:
Deposited by: Yamamoto, T.
Restriction:
Photo: