細胞株の詳細データ

TKG 0321 :: RBL 2H3

ID TKG 0321
Cell name RBL 2H3
Data accepted Sep., 1990.
Animal Rat.
Scientific name Rattus norvegicus.
Sex
Case history
Tissue Peripheral blood.
Genetics
Life span Infinite.
Morphology Spindle-forming (fibroblastic).
Medium MEM + 10% FBS (RPMI-1640 + 10% FBS).
Passage method 0.02% EDTA-PBS and 0.25% trypsin.
Characteristics Basophilic lelukemia cell line, releasing histamine by stimulation of IgE recepror.
Temperature 37℃
CO2 concentration 5%
Subculture frequency 1:20, twice a week.
Classification
Mycoplasma PCR: -, Hoechst DNA staining: -
Isozyme analysis LD
Established by Siraganian, R. & Eccleston.
References Nature New Biol., 224, 73-76, 1973. Fed. Proc., 11, 317-323, 1979.
References by users Biosci. Biotech. Biochem., 61, 19-23, 1997(PMID: 9028031).
Comments
Deposited by Maeyama, K. & Tanno, Y.
Restriction
Photo
 Photo(x100)