細胞株の詳細データ

TKG 0331 ：： HeLa

ID	TKG 0331
Cell name	HeLa
Data accepted	Aug., 1989.
Animal	Human.
Scientific name	Homo sapiens.
Sex
Case history
Tissue	Cervix (epitheloid carcinoma).
Genetics
Life span	Infinite.
Morphology	Epithelial-like
Medium	MEM + 10% FBS.
Passage method	0.25% trypsin.
Characteristics	The first aneupleud, epithelial-like cell line derived from human tissue (31 y. o. Negro female). Recent histological study of original slide revealed that tumor was adenocarcinoma. 　A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210.
Temperature	37℃
Survival
CO2 concentration	5%
Subculture frequency	1:10～1:20, twice a week.
Classification
Mycoplasma	PCR: -, Hoechst DNA staining: -
Established by	Gey, G. O. (1951).
References	Cancer Res., 12, 264, 1952. J. Exp. Med., 97, 695, 1953(PMID: 13052828)
References by users	Biol. Pharm. Bull., 24, 1338-1341, 2001(PMID: 11725977)., Biol. Pharm. Bull., 24, 1443-1445, 2001(PMID: 11767121)., Biol. Pharm. Bull., 25, 875-879, 2002(PMID: 12132661)., Biol. Pharm. Bull., 25, 920-922, 2002(PMID: 12132670)., Int. J. Oncol., 20, 943-948, 2002(PMID: 11956587)., J. Bio. Chem., 277, 26252-26259, 2002(PMID: 12011038)., Natural Med., 56, 136-138, 2002., Toxicology Letters, 143, 1-7, 2003(PMID: 12697374)., Biochem. Biophys. Res. Commun., 346, 484-490, 2006(PMID: 16765317), Cancer Med. 2014 Apr;3(2):310-21(PMID: 24464839), Biochem Biophys Res Commun. 2017 Aug 19;490(2):330-335(PMID: 28619507), Mol Biol Rep. 2020 Jun;47(6):4401-4411(PMID: 32394308), J Radiat Res. 2020 Nov 16;61(6):819-827(PMID: 32880638), Technol Cancer Res Treat. 2020 Jan-Dec:19:1533033820980077(PMID: 33334271), Int J Mol Sci. 2021 Aug 2;22(15):8300(PMID: 34361070).
Comments
Deposited by	Shibahara, S. (Tohoku U.).
Restriction
DNA Profile (STR-PCR)	D5S818 D13S317 D7S820 D16S539 vWA TH01 Amelogenin TPOX CSF1PO 11, 12 12, 13.3 8, 12 9, 10 16, 18 7 X 8, 12 9, 10
Photo