細胞株の詳細データ

TKG 0331 :: HeLa

ID TKG 0331
Cell name HeLa
Data accepted Aug., 1989.
Animal Human.
Scientific name Homo sapiens.
Sex
Case history
Tissue Cervix (epitheloid carcinoma).
Genetics
Life span Infinite.
Morphology Epithelial-like
Medium MEM + 10% FBS.
Passage method 0.25% trypsin.
Characteristics The first aneupleud, epithelial-like cell line derived from human tissue (31 y. o. Negro female). Recent histological study of original slide revealed that tumor was adenocarcinoma.  A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210.
Temperature 37℃
Survival
CO2 concentration 5%
Subculture frequency 1:10~1:20, twice a week.
Classification
Mycoplasma PCR: -, Hoechst DNA staining: -
Established by Gey, G. O. (1951).
References Cancer Res., 12, 264, 1952. J. Exp. Med., 97, 695, 1953(PMID: 13052828)
References by users Biol. Pharm. Bull., 24, 1338-1341, 2001(PMID: 11725977)., Biol. Pharm. Bull., 24, 1443-1445, 2001(PMID: 11767121)., Biol. Pharm. Bull., 25, 875-879, 2002(PMID: 12132661)., Biol. Pharm. Bull., 25, 920-922, 2002(PMID: 12132670)., Int. J. Oncol., 20, 943-948, 2002(PMID: 11956587)., J. Bio. Chem., 277, 26252-26259, 2002(PMID: 12011038)., Natural Med., 56, 136-138, 2002., Toxicology Letters, 143, 1-7, 2003(PMID: 12697374)., Biochem. Biophys. Res. Commun., 346, 484-490, 2006(PMID: 16765317), Cancer Med. 2014 Apr;3(2):310-21(PMID: 24464839), Biochem Biophys Res Commun. 2017 Aug 19;490(2):330-335(PMID: 28619507), Mol Biol Rep. 2020 Jun;47(6):4401-4411(PMID: 32394308), J Radiat Res. 2020 Nov 16;61(6):819-827(PMID: 32880638), Technol Cancer Res Treat. 2020 Jan-Dec:19:1533033820980077(PMID: 33334271), Int J Mol Sci. 2021 Aug 2;22(15):8300(PMID: 34361070).
Comments
Deposited by Shibahara, S. (Tohoku U.).
Restriction
DNA Profile
(STR-PCR)
D5S818 D13S317 D7S820 D16S539 vWA TH01 Amelogenin TPOX CSF1PO
11, 12 12, 13.3 8, 12 9, 10 16, 18 7 X 8, 12 9, 10
Photo