細胞株の詳細データ

TKG 0331 :: Hela

ID: TKG 0331
Cell name: HeLa
Data accepted: Aug., 1989.
Animal: Human.
Scientific name: Homo sapiens.
Sex:
Case history:
Tissue: Cervix (epitheloid carcinoma).
Genetics:
Life span: Infinite.
Morphology: Epithelial-like
Medium: MEM + 10% FBS.
Passage method: 0.25% trypsin.
Characteristics: The first aneupleud, epithelial-like cell line derived from human tissue (31 y. o. Negro female). Recent histological study of original slide revealed that tumor was adenocarcinoma.  A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210.
Temperature: 37℃
Survival
CO2 concentration: 5%
Subculture frequency: 1:10~1:20, twice a week.
Classification:
Mycoplasma: PCR: -, Hoechst DNA staining: -
Established by: Gey, G. O. (1951).
References: Cancer Res., 12, 264, 1952. J. Exp. Med., 97, 695, 1953(PMID: 13052828)
References by users: Biol. Pharm. Bull., 24, 1338-1341, 2001(PMID: 11725977)., Biol. Pharm. Bull., 24, 1443-1445, 2001(PMID: 11767121)., Biol. Pharm. Bull., 25, 875-879, 2002(PMID: 12132661)., Biol. Pharm. Bull., 25, 920-922, 2002(PMID: 12132670)., Int. J. Oncol., 20, 943-948, 2002(PMID: 11956587)., J. Bio. Chem., 277, 26252-26259, 2002(PMID: 12011038)., Natural Med., 56, 136-138, 2002., Toxicology Letters, 143, 1-7, 2003(PMID: 12697374)., Biochem. Biophys. Res. Commun., 346, 484-490, 2006(PMID: 16765317).
Comments:
Deposited by: Shibahara, S. (Tohoku U.).
Restriction:
DNA Profile
(STR-PCR)
D5S818 D13S317 D7S820 D16S539 vWA TH01 Amelogenin TPOX CSF1PO
11, 12 12, 13.3 8, 12 9, 10 16, 18 7 X 8, 12 9, 10
Photo: