| ID |
TKG 0331 |
| Cell name |
HeLa |
| Data accepted |
Aug., 1989. |
| Animal |
Human. |
| Scientific name |
Homo sapiens. |
| Sex |
|
| Case history |
|
| Tissue |
Cervix (epitheloid carcinoma).
|
| Genetics |
|
| Life span |
Infinite. |
| Morphology |
Epithelial-like |
| Medium |
MEM + 10% FBS.
|
| Passage method |
0.25% trypsin. |
| Characteristics |
The first aneupleud, epithelial-like cell line derived from human tissue
(31 y. o. Negro female). Recent histological study of original slide revealed
that tumor was adenocarcinoma. A culture submitted to the Cell Resource
Center for Biomedical Research was found to be contaminated with mycoplasma.
Progeny were cured by Treatments with BM Cyclin and MC210. |
| Temperature |
37℃ |
| Survival |
|
| CO2 concentration |
5% |
| Subculture frequency |
1:10～1:20, twice a week. |
| Classification |
|
| Mycoplasma |
PCR: -, Hoechst DNA staining: - |
| Established by |
Gey, G. O. (1951).
|
| References |
Cancer Res., 12, 264, 1952. J. Exp. Med., 97, 695, 1953(PMID: 13052828) |
| References by users |
Biol. Pharm. Bull., 24, 1338-1341, 2001(PMID: 11725977)., Biol. Pharm. Bull., 24, 1443-1445, 2001(PMID: 11767121)., Biol. Pharm. Bull., 25, 875-879, 2002(PMID: 12132661)., Biol. Pharm. Bull., 25, 920-922, 2002(PMID: 12132670)., Int. J. Oncol., 20, 943-948, 2002(PMID: 11956587)., J. Bio. Chem., 277, 26252-26259, 2002(PMID: 12011038)., Natural Med., 56, 136-138, 2002., Toxicology Letters, 143, 1-7, 2003(PMID:
12697374)., Biochem. Biophys. Res. Commun., 346, 484-490, 2006(PMID: 16765317). |
| Comments |
|
| Deposited by |
Shibahara, S. (Tohoku U.). |
| Restriction |
|
DNA Profile
(STR-PCR) |
| D5S818 |
D13S317 |
D7S820 |
D16S539 |
vWA |
TH01 |
Amelogenin |
TPOX |
CSF1PO |
| 11, 12 |
12, 13.3 |
8, 12 |
9, 10 |
16, 18 |
7 |
X |
8, 12 |
9, 10 |
|
| Photo |
 |
