細胞株の詳細データ

TKG 0345 :: HL-60

ID TKG 0345
Cell name HL-60
Data accepted June 29, 1988.
Animal Human.
Scientific name Homo sapiens.
Sex
Case history
Tissue Periph, blood premyelocytic leukemia.
Genetics
Life span Infinite.
Morphology Lymphoblast-like.
Medium RPMI-1640 + 10% FBS.
Passage method Dilution
Characteristics Obtained from blood of 36 y. o. Caucasion female, acute promyelocytic leukemia. Variety of drugs including DMSO, butylate, hypoxanthine, TPA, retinoic acid and actinomycin D induce differenciation.
Temperature 37℃
CO2 concentration 5%
Subculture frequency 1:2~1:4, twice a week.
Classification
Mycoplasma PCR: -, Hoechst DNA staining: -
Established by Collins, S. J.
References Nature, 270, 347-349, 1977(PMID: 271272)., Blood, 54, 713-733, 1979(PMID: 288488).
References by users Arch. Biochem. and Biophy., 418, 99-108, 2003(PMID: 14522581)., Int. J. Oncol., 23, 705-712, 2003(PMID: 12888907), Basic Clin Pharmacol Toxicol. 2006 Jan;98(1):44-50(PMID: 16433890), Acta Biochim Pol. 2007;54(4):777-82(PMID: 18066407), Technol Health Care. 2018;26(1):93-108(PMID: 29309044), .
Comments
Deposited by Dep. Clin. Cancer Chemoth., (RITC).
Restriction
DNA Profile
(STR-PCR)
D5S818 D13S317 D7S820 D16S539 vWA TH01 Amelogenin TPOX CSF1PO
12 8, 11 11, 12 11 16 7, 8 X 8, 11 13, 14
Photo