細胞株の詳細データ

TKG 0402 :: KG-1a

ID: TKG 0402
Cell name: KG-1a
Data accepted:
Animal: Human.
Scientific name: Homo sapiens.
Sex:
Case history:
Tissue: Bone marrow.
Genetics:
Life span: Infinite.
Morphology: Lymphoblast-like.
Medium: RPMI-1640 + 10% FBS.
Passage method: Dilute 1/5 every 3-4 days.
Characteristics: The variant subline of KG-1 cell line. The variant KG-1a cell line was separated from parent KG-1 on the basis of morphological difference after 35 passages. KG-1a is unresponsive to colony-stimulating factor in soft-agar culture and does not express Ia-Ag. KG-1a cells are resistant to phorbol diester macrophage differenciation and proliferation of the cells is unaffected by the presence of phorbol diesters. This cell line is morphologically, cytochemically, and functionally less mature than the parental KG-1. Human AML cell line.  A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210.
Temperature: 37℃
Survival
CO2 concentration: 5%
Subculture frequency:
Classification:
Mycoplasma: PCR: -, Hoechst DNA staining: -
Established by: Koeffler, H. P.
References: Blood, 56, 265-273, 1980(PMID: 6967340)., Blood, 62, 709-721, 1983(PMID: 6192859).
References by users: J. Gastroenterol. Hepatol., 21, 1334-1339, 2006(PMID: 16872319).
Comments:
Deposited by:
Restriction:
DNA Profile
(STR-PCR)
D5S818 D13S317 D7S820 D16S539 vWA TH01 Amelogenin TPOX CSF1PO
13 11, 12 8, 10 10, 11 14, 19 7, 8 XY 7, 9 7
Photo: x100