細胞株の詳細データ

TKG 0438 :: HuH-28

ID: TKG 0438
Cell name: HuH-28
Data accepted: June, 1995.
Animal: Human.
Scientific name: Homo sapiens.
Sex:
Case history:
Tissue: Cholangiocellular carcinoma (intrahepatic).
Genetics:
Life span: Infinite.
Morphology: Spindle-shaped with small percentage of poly-gonal-shaped cells.
Medium: MEM + 10% FBS.
Passage method: 0.25% Trypsin.
Characteristics: Slow growing, doubling time is about 80 h. Not transplantable to nude mouse. Alkaline phosphatase (+), Gamma GTP (+), beta microglubulin (+), ferritin (+), Elastase-1 (+), tissue peptide antigen (+).  A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210.
Temperature: 37℃
Survival
CO2 concentration: 5%
Subculture frequency: 1:1~1:2, twice a week.
Classification:
Mycoplasma: PCR: -, Hoechst DNA staining: -
Established by: Y. Kusaka.
References: Human cell (Japanese), 1, 92-94, 1988(PMID: 2856443)., Res. Exp. Med. Berl., 188, 367-375, 1988(PMID: 2852388).
References by users: Int. J. Oncol., 36, 245-254, 2010(PMID: 19956853).
Comments:
Deposited by:
Restriction:
DNA Profile
(STR-PCR)
D5S818 D13S317 D7S820 D16S539 vWA TH01 Amelogenin TPOX CSF1PO
9, 12 9, 12 10, 11 9 17 9 X 8 9, 12
Photo: (x100)