細胞株の詳細データ

TKG 0478 :: L1210 TGR4

ID TKG 0478
Cell name L1210 TGR4
Data accepted April, 1996.
Animal DBA/2 mouse.
Scientific name Mus musculus.
Sex
Case history
Tissue Leukemia.
Genetics
Life span Infinite.
Morphology Lymphoblastic.
Medium RPMI-1640 + 10% FBS, 10 μg 6TG/ml).
Passage method Dilute 1/4 every 3-4 days.
Characteristics This line was selected by the treatment of EMS from L1210. Resistant to 6TG (10 microgram/ml). This line is more immunogenic than the parent line and incapable of forming ascites tumors in BDF1 mice. 86 KDa (p86) protein, specific to this line, was identified as tumor rejection antigen by the studies of cell fusion between L1210 TGR4 and parent line. Immune-response directed to the specific p86 on L1210 TGR4 may be involved in the loss of tumorigenicity of this line.
CO2 concentration 5%
Subculture frequency
Classification
Mycoplasma PCR: -, Hoechst DNA staining: -
Isozyme analysis LD
Established by Noriko Yamada (1st. Biochem. Kanazawa Medical Univ. Uchinada, Ishikawa).
References 4st Int. Congress of Cell Biol. Aug. 1988. Abstract, p46.
References by users
Comments
Deposited by Noriko Yamada
Restriction
Photo