細胞株の詳細データ

TKG 0482 :: KMST-6

ID: TKG 0482
Cell name: KMST-6
Data accepted:
Animal: Human.
Scientific name: Homo sapiens
Sex:
Case history:
Tissue: Embryonal fibroblas.
Genetics:
Life span: Infinite
Morphology: Fibroblast-like
Medium: E-MEM + 10% FBS or RPMI 1640 + 10% FBS
Passage method: 0.25% trypsin
Characteristics: A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210
Misc: Characteristics Embryonal fibroblast immortalized by 60-Co irradiation (400 Rad x 1 time and 200 rad x 12 times). Non-tumorigenic.
Survival
CO2 concentration: 5%
Subculture frequency: 1:10, twice a week.
Classification:
Mycoplasma: PCR: -, Hoechst DNA staining: -
Established by: Namba, M.
References: Human Cell, 6, 253-259, 1993(PMID: 8148305)., J. Cancer Res. Clin. Oncol., 119, 522-526, 1993(PMID: 8100822)., Mol. Cell. Biol., 13, 6036-6043, 1993(PMID: 8105370)., Human Cell, 7, 88-94, 1994(PMID: 8279474)., In Vitro Cell Dev. Biol. Anim.,, 30A, 199-201, 1994(PMID: 7520808)., Cell Struct. Funct., 20, 41-45, 1995(PMID: 7540958)., Electrophoresis, 16, 1067-1073, 1995(PMID: 7498129)., "Senescence, Immortalization, Transformation." Ed. Namba, Watanabe & Hayflick, Kyoritus Pub. (ISBN4-320-06107-1), 1995. Jap. J. Cancer Res., 86, 35-40, 1995(PMID: 7737907)., Human Genet., 97, 1-6, 1996(PMID: 8557247)., J. Cancer Res. Clinic. Oncol., 122, 249-253, 1996(PMID: 8601579)., In Vitro Cell Dev. Biol. Anim., 34, 463-467, 1998(PMID: 9661049)..
This cell line is supplied by Health Science Research Resources Bank (HSRRB).
References by users: Clin. Cancer Res., 11, 6800-6806, 2005(PMID: 16203767).
Comments:
Deposited by:
Restriction:
DNA Profile
(STR-PCR)
D5S818 D13S317 D7S820 D16S539 vWA TH01 Amelogenin TPOX CSF1PO
10, 12 10, 11 9 11 14, 18 9 X 11, 12 12
Photo: (x100)