細胞株の詳細データ

TKG 0493 :: PK-45P

ID TKG 0493
Cell name PK-45P
Data accepted Nov. 1996.
Animal Human.
Scientific name Homo sapiens.
Sex
Case history
Tissue Pancreas.
Genetics
Life span Infinite.
Morphology Epithelial-like.partly spindle cell-like.
Medium RPMI-1640 + 10% FBS.
Passage method 0.25% trypsin.
Characteristics Pancreatic carcinoma.  A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cycline and MC210.
CO2 concentration 5%
Subculture frequency 1:10, twice a week.
Classification
Mycoplasma PCR: -, Hoechst DNA staining: -
Isozyme analysis LD
Established by Masao Kobari (1st Dept. Surgery, Tohoku Univ.).
References
References by users Cancer Letters, 176, 199-203, 2002(PMID: 11804748)., Cancer Res., 67, 9704-9712, 2007(PMID: 17942900)., J. Surg. Oncol., 103, 230-238, 2011(PMID: 21337550).
Comments
Deposited by M. Kobari.
Restriction
DNA Profile
(STR-PCR)
D5S818 D13S317 D7S820 D16S539 vWA TH01 Amelogenin TPOX CSF1PO
10, 12 9, 11 11 11, 12 18 9 X 11 9, 10
Photo   
 Photo(x100)                       Photo(x100)