| ID |
TKG 0504 |
| Cell name |
HCT-15 |
| Data accepted |
|
| Animal |
Human. |
| Scientific name |
Homo sapiens |
| Sex |
|
| Case history |
|
| Tissue |
Colon, Adenocarcinoma. |
| Genetics |
|
| Life span |
Infinite. |
| Morphology |
Epithelial-like. |
| Medium |
RPMI-1640 + 10% FBS. |
| Passage method |
0.02% EDTA-PBS. |
| Characteristics |
Human colorectal adenocarcinoma. Blood 0 type. CEA production, 5.4 ng/ml in 10
days. Transplantable to nude mouse. Evidence from DNA fingerprinting indicates
that this line and DLD-1 are derived from the same individual. |
| CO2 concentration |
5% |
| Subculture frequency |
1:4 ~ 1:9, twice a week. |
| Classification |
|
| Mycoplasma |
PCR: -, Hoechst DNA staining: - |
| Established by |
Dexter, D.L. et al. |
| References |
Cancer Res., 39, 1020-1025, 1979(PMID: 427742). |
| References by users |
Proc. Natl. Acad. Sci., 99, 10718-10723, 2002(PMID: 12149448)., Toxicology Letters, 143, 1-7, 2003(PMID: 12697374)., J. Biol. Chem., 279, 27315-27319, 2004(PMID: 15102855)., Cancer Sci., 98, 299-307, 2007(PMID: 17270019)., Proc. Natl. Acad. Sci., 107, 17309-17314, 2010(PMID: 20855608). |
| Comments |
|
| Deposited by |
|
| Restriction |
|
DNA Profile
(STR-PCR) |
| D5S818 |
D13S317 |
D7S820 |
D16S539 |
vWA |
TH01 |
Amelogenin |
TPOX |
CSF1PO |
| 13 |
8, 11 |
10, 12 |
12, 13 |
18, 19 |
7, 9.3 |
XY |
8, 11 |
12 |
|
| Photo |
 |
