細胞株の詳細データ

TKG 0519 :: F9

ID: TKG 0519
Cell name: F9
Data accepted:
Animal: 129 mouse
Scientific name: Mus musculus
Sex:
Case history:
Tissue: Embryonal carcinoma
Genetics:
Life span: Infinite
Morphology: Polymorphic
Medium: D-MEM + 15% FBS or RPMI-1640 + 10% FBS
Passage method: 0.02% EDTA-PBS or 0.05% trypsin, RT
Characteristics: This cell line was established from testicular terato-carcinoma of a strain 129 mouse. This shows limited differenciation under normal culture conditions, can be induced to differenciate into partial endoderm in the presence of retinoic acid and dibutyric cyclic AMP. The newphenotype is characterized by synthesis of plasminogen activator, laminin and type IV collagen and by low levels of alkali phosphatase and lactate dehydrogenase. The cell type generated by this induction is not dependent upon the continuous presence of either compounds, but the cAMP agents are active only F9 cells the have been treated with retinoic acid. (The F9 cells are routinely propagated on culture surfaces that have been treated with 0.1% gelatin). A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210.
CO2 concentration: 5%
Subculture frequency: 1:40~1:80, twice a week.
Classification:
Mycoplasma: PCR: -, Hoechst DNA staining: -
Isozyme analysis: LD
Established by: Bernstine et al.
References: ProNas.,70, 3899-3903, 1973.PMID: 4521215 Cell, 15, 393-403, 1978.PMID: 214238 Cell, 21, 347-355, 1980.PMID: 6250719
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