細胞株の詳細データ

TKG 0567 :: BeWo

ID: TKG 0567
Cell name: BeWo
Data accepted:
Animal: Human.
Scientific name:
Sex:
Case history:
Tissue: Fetal placenta
Genetics:
Life span: Infinite.
Morphology: Epithelial-like.
Medium: F12 85% and 15% FBS or RPMI 1640 45% +5% FBS, and 50% GIT medium(Wako)
Passage method: 0.02% EDTA-PBS.
Characteristics: Human choriocarcinoma (trophoblastic cell line) The tumor was originally transplanted to the hamster cheek pouch by R. Hertz where it was maintained for 304 serial transfers over a period of 8 years before establishment in vitro. The BeWo cell line has been shown to secrete hCG, polypeptide hormone, estrogen and estradiol.
CO2 concentration: 5%
Subculture frequency: 1:4, twice a week.
Classification:
Mycoplasma: PCR: -, Hoechst DNA staining: -
Isozyme analysis:
Established by: Pattillo, R.A. and Gey, G.O.
References: Proc. Soc. Exp. Biol. Med., 102, 77, 1959(PMID: 14401422)., Cancer Res., 28, 1231-1236, 1968(PMID: 4299001)., Science, 159, 1467-1469, 1968(PMID: 5753554)., Ann. N. Y. Acad. Sci., 172, 288-298, 1971(PMID: 5289994).,. J. Clin. Endocrinol. Metab., 34, 59-61, 1972(PMID: 4332667).
References by users: Cancer Res., 67, 3945-3954, 2007(PMID: 17440110).
Comments:
Deposited by:
Restriction:
DNA Profile
(STR-PCR)
D5S818 D13S317 D7S820 D16S539 vWA TH01 Amelogenin TPOX CSF1PO
10, 11 9, 11 10, 12 13, 14 16 9, 9.3 XY 8 11, 12
Photo: