| ID |
TKG 0604 |
| Cell name |
DU145 |
| Data accepted |
|
| Animal |
Human |
| Scientific name |
Homo sapiens. |
| Sex |
|
| Case history |
|
| Tissue |
Prostate carcinoma brain metastasis. |
| Genetics |
|
| Life span |
Infinite. |
| Morphology |
Epithelial-like. |
| Medium |
RPMI-1640 plus 10% FBS or Eagle's MEM +10% FBS |
| Passage method |
0.05% trypsin-0.02% EDTA/PBS. |
| Characteristics |
This cell line was established from a lesion in the brain of a patient
(69 year-old, Caucasian, blood type O) with widespread brain metastasis
of prostate carcinoma and a 3-year history of lymphocyteic leukemia. Tumorigenic
in nude mouse. |
| CO2 concentration |
5% |
| Subculture frequency |
1:4, twice a week. |
| Classification |
|
| Mycoplasma |
PCR: -, Hoechst DNA staining: - |
| Established by |
K.R. Stone |
| References |
Cancer Res., 37, 4049-4058, 1977.PMID: 908039, Int. J. Cancer, 21, 274-281, 1978.PMID: 631930 |
| References by users |
Neoplasma. 2012;59(2):224-32(PMID: 22248281), Mol Cell Biol. 2015 Oct 19;36(1):144-56. (PMID: 26483416) Cancer Sci. 2021 Sep;112(9):3871-3883(PMID: 34050700), Life Sci. 2022 Jan 1:288:120171(PMID: 34822800). |
| Comments |
|
| Deposited by |
|
| Restriction |
|
DNA Profile
(STR-PCR) |
| D5S818 |
D13S317 |
D7S820 |
D16S539 |
vWA |
TH01 |
Amelogenin |
TPOX |
CSF1PO |
| 10,13 |
12,14 |
7,10,11 |
11,13 |
17,18 |
7 |
XY |
11 |
10,11 |
|
| Photo |
 (x100) |
