細胞株の詳細データ

TKG 0721 :: Hepa1-3xFlag-AhR-HA-pCEP4(CAG)H12C1

ID TKG 0721
Cell name Hepa1-3xFlag-AhR-HA-pCEP4(CAG)H12C1
Data accepted March, 2014
Animal Mouse C56BL
Scientific name Mus musculus.
Sex
Case history
Tissue Liver
Genetics Stable Hepa1c1c7 cl19E4 mutant transfected with 3xFlag-HA-tagged aryl hydrocarbon receptor (AhR) gene.
Life span Infinite
Morphology Fibroblast-like.
Medium DMEM + 5% FBS
Passage method 0.25% trypsin/EDTA
Characteristics Resistant to 150㎍/ml hygromycin B. Useful for analysis of AhR transcription factor complex.
A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210.
Temperature 37℃
CO2 concentration 5%
Subculture frequency 1:10, twice a week.
Classification
Mycoplasma PCR: -, Hoechst DNA staining: -
Established by Ebina, M.
References Ebina M, et al. Biochim. Biophys.Acta,1809,176 (2011)
References by users
Comments
Deposited by Kikuchi, H.
Restriction
Photo