細胞株の詳細データ

TKG 0725 :: Seki

ID TKG 0725
Cell name Seki
Data accepted December, 2014
Animal Human.
Scientific name Homo sapiens.
Sex M
Case history
Tissue Ewing's sarcoma.
Genetics Translocation of t(11;22)(q24;q12), EWSR1-Fli1.
Life span Infinite.
Morphology
Medium RPMI1640 + 10% FBS.
Passage method 0.05% trypsin/EDTA, collagen coated dish.
Characteristics Transplantable tumor cell line (subcutaneously, Nude mouse, SCID).
Temperature 37℃
CO2 concentration 5%
Subculture frequency 1:5-1:10, twice a week.
Classification
Mycoplasma PCR: -, Hoechst DNA staining: ±
Established by Nojima, T.
References Igaku no ayumi. 145(7):511-512. Human Cell. 2:192-193(PMID: 2486607). Nagoya Med J. 35:169-180. Genes Chromosome Cancer. 13:126-132(PMID: 7542907).
References by users
Comments
Deposited by Nojima, T.
Restriction
DNA Profile
(STR-PCR)
D5S818 D13S317 D7S820 D16S539 vWA TH01 Amelogenin TPOX CSF1PO
10, 12 8, 12 10, 13 12, 13 14, 18 6, 9 XY 8 10, 12
Photo