細胞株の詳細データ

TKG 0750:: Replic

ID TKG 0750
Cell name Replic
Data accepted August, 2019.
Animal Mouse, C57BL/6
Scientific name Mus musculus.
Sex Male.
Case history
Tissue Kidney, REP (renal erythropoietin-producing) cells.
Genetics
Life span Infinite.
Morphology Fibroblast-like
Medium DMEM high glucose+ 10% FBS
Passage method Accutase (Nakarai 12679-54) or 0.05% trypsin.
Characteristics The immortalized cell line prepared by the exogenous expression of oncogenic H-RAS (G12V).
tdTomato-positive. High-level TGFβ expression.
This cell line was isolated from Epo(GFP/wt)Tg(3.3kEpo)Tg(EpoCre)Rosa26(LSLtdTomato) genotype mice.
A culture submitted to Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by treatments with BM Cyclin and MC210.
Temperature 37℃
CO2 concentration 5%
Subculture frequency 1:5 ~ 1:10, twicw a week
Classification
Mycoplasma PCR: -, Hoechst DNA staining: -
Established by Suzuki, N.
References Sci Rep 9(1):11254(2019). PMID: 31375751.
References by users
Comments
Deposited by Suzuki,N.
Restriction C: A prior written permission of the originator is necessary.
Photo