細胞株の詳細データ
TKG 0752：： mTX3
| ID | TKG 0752 |
| Cell name | mTX3 |
| Data accepted | January, 2019. |
| Animal | Mouse x Rat |
| Scientific name | |
| Sex | |
| Case history | |
| Tissue | B cell hybridoma. |
| Genetics | |
| Life span | |
| Morphology | Lymphoblast-like. |
| Medium | RPMI1640 + 10% FBS |
| Passage method | Dilution. |
| Characteristics | Immune animal is Wistar rat. Antigen is mouse testicular homogenate named
TEX101. Mouse-Rat hybridoma producing rat monoclonal antibody, IgG2a, kappa.
HAT resistance. This cell line can be used for immunoprecipitation, flow
cytometry and non-reducing western blotting. It may also be used for immunohistochemical
staining. Transplantation into C.B-17/Icr-scid/scid mice induces ascites. |
| Temperature | 37℃ |
| CO2 concentration | 5% |
| Subculture frequency | 1:10 ~ 1:20, twice a week. |
| Classification | |
| Mycoplasma | PCR: -, Hoechst DNA staining: - |
| Established by | Tsukamoto, H. |
| References | Sci Rep.2016 6:23616 (PMID: 270005865) |
| References by users | |
| Comments | |
| Deposited by | Tsukamoto, H. |
| Restriction | C: A prior written permission of the originator is necessary. |
| Photo |   (x100) (x200) |