細胞株の詳細データ

TKG 0753 ::mTX4

ID TKG 0753
Cell name mTX4
Data accepted January, 2019.
Animal Mouse x Rat
Scientific name
Sex
Case history
Tissue B cell hybridoma.
Genetics
Life span
Morphology Lymphoblast-like.
Medium RPMI1640 + 10% FBS
Passage method Dilution.
Characteristics Immune animal is Wistar rat. Antigen is mouse testicular homogenate named TEX101. Mouse-Rat hybridoma producing rat monoclonal antibody, IgG2a, kappa. HAT resistance. This cell line can be used for immunoprecipitation, flow cytometry and non-reducing western blotting. It may also be used for immunohistochemical staining. Transplantation into C.B-17/Icr-scid/scid mice induces ascites.
Temperature 37℃
CO2 concentration 5%
Subculture frequency 1:10 ~ 1:20, twice a week.
Classification
Mycoplasma PCR: -, Hoechst DNA staining: -
Established by Tsukamoto, H.
References Sci Rep.2016 6:23616 (PMID:27005865)
References by users
Comments
Deposited by Tsukamoto, H.
Restriction C: A prior written permission of the originator is necessary.
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