細胞株の詳細データ

TKG 0761:: 1F1

ID TKG 0761
Cell name 1F1
Data accepted January, 2019
Animal Mouse x Rat
Scientific name
Sex
Case history
Tissue B cell hybridoma.
Genetics
Life span
Morphology Lymphoblast-like.
Medium RPMI1640 + 10% FBS
Passage method Dilution.
Characteristics Wistar rats were immunized with human CD14-expressing NRK-52E transfected cells. Mouse - Rat hybridoma producing rat monoclonal antibody, IgG2a, kappa. It doesn't cross-react with mouse CD14.
HAT resistance. This cell line can be used for immunoprecipitation and flow cytometry. Transplantation into C.B-17/Icr-scid/scid mice induces ascites.
Temperature 37℃
CO2 concentration 5%
Subculture frequency 1:4, twice a week.
Classification
Mycoplasma PCR: -, Hoechst DNA staining: -
Established by Tsukamoto, H.
References J Immunol. 2013 190(1):195-204 (PMID: 23203928).
References by users
Comments
Deposited by Tsukamoto, H.
Restriction C: A prior written permission of the originator is necessary.
Photo