細胞株の詳細データ

TKG 0765:: GFP5

ID TKG 0765
Cell name GFP5
Data accepted January, 2019.
Animal Mouse x Rat
Scientific name
Sex
Case history
Tissue B cell hybridoma
Genetics
Life span
Morphology Lymphoblast-like
Medium RPMI1640 + 10% FBS
Passage method Dilution.
Characteristics Wistar rats were immunized in the foot pads with EGFP. Mouse- Rat hybridoma producing rat monoclonal antibody, IgG2a, kappa. HAT resistance. This cell line can be used for immunoprecipitation for antigen with EGFP in C-terminal and FACS. Transplantation into C.B-17/Icr-scid/scid mice induces ascites.
Temperature 37℃
CO2 concentration 5%
Subculture frequency 1:4 ~ 1:9, twice a week.
Classification
Mycoplasma PCR: -, Hoechst DNA staining: -
Established by Tsukamoto, H.
References J Biol Chem.2018 293(26):10186-10201 (PMID: 29760187).
References by users
Comments
Deposited by Tsukamoto, H.
Restriction C: A prior written permission of the originator is necessary.
Photo