細胞株の詳細データ

TKG 0772:: HT26

ID TKG 0772
Cell name HT26
Data accepted February, 2019.
Animal Mouse x Mouse
Scientific name
Sex
Case history
Tissue B cell hybridoma
Genetics
Life span
Morphology Lymphoblast-like
Medium RPMI1640 + 10% FBS
Passage method Dilution.
Characteristics TLR4-/- mice on C57BL/6 genetic background were immunized with Ba/F3-transfected cells expressing human TLR4/MD-2. Mouse - Mouse hybridoma producing mouse monoclonal antibody, IgG1, kappa. Human TLR4/MD-2 complex-specific. It doesn't react with human TLR4 and MD-2. No cross-reactivity with mouse TLR4/MD-2.
HAT resistance. This cell line can be used for immunoprecipitation and flow cytometry. Transplantation into C.B-17/Icr-scid/scid mice induces ascites.
Temperature 37℃
CO2 concentration 5%
Subculture frequency 1:1 ~ 1:4, twice a week.
Classification
Mycoplasma PCR: -, Hoechst DNA staining: -
Established by Tsukamoto, H.
References Int Immunol. 2012 24(8):495-506(PMID: 22499954).
Biochem Biophys Res Commun. 2013 440(1):31-6(PMID: 24021278).
FEBS Lett. 2015 589(24 Pt B):3893-8(PMID: 26602081).
FEBS Lett. 2017 591(16):2406-2416(PMID: 28741733).
References by users
Comments
Deposited by Tsukamoto, H.
Restriction C: A prior written permission of the originator is necessary.
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