ID	TKG 0790
Cell name	M14-9
Data accepted	February, 2019.
Animal	Mouse x Rat
Scientific name
Sex
Case history
Tissue	B cell hybridoma
Genetics
Life span
Morphology	Lymphoblast-like
Medium	RPMI1640 + 10% FBS
Passage method	Dilution.
Characteristics	Wistar rats were immunized with mouse CD14-expressing NRK-52E transfected cells. Mouse - Rat hybridoma producing rat monoclonal antibody, IgG2a, kappa. HAT resistance. This cell line can be used for immunoprecipitation and flow cytometry. It doesn't inhibit LPS binding of CD14. No cross-reactivity with human CD14. Transplantation into C.B-17/Icr-scid/scid mice induces ascites.
Temperature	37℃
CO2 concentration	5%
Subculture frequency	1:4, twice a week.
Classification
Mycoplasma	PCR: -, Hoechst DNA staining: -
Established by	Tsukamoto, H.
References	Int Immunol. 2010 22(4):271-80(PMID: 20133493).
References by users
Comments
Deposited by	Tsukamoto, H.
Restriction	C: A prior written permission of the originator is necessary.
Photo