細胞株の詳細データ

TKG 0796:: M14-21

ID TKG 0796
Cell name M14-21
Data accepted February, 2019.
Animal Mouse x Rat
Scientific name
Sex
Case history
Tissue B cell hybridoma.
Genetics
Life span
Morphology Lymphoblast-like
Medium RPMI1640 + 10% FBS
Passage method Dilution.
Characteristics Wistar rats were immunized with mouse CD14-expressing NRK-52E transfected cells. Mouse - Rat hybridoma producing rat monoclonal antibody, IgG2a. HAT resistance. This cell line can be used for immunoprecipitation and flow cytometry.
It weakly inhibits LPS binding of CD14. No cross-reactivity with human CD14. Transplantation into C.B-17/Icr-scid/scid mice induces ascites.
Temperature 37℃
CO2 concentration 5%
Subculture frequency 1:4, twice a week.
Classification
Mycoplasma PCR: -, Hoechst DNA staining: -
Established by Tsukamoto, H.
References Int Immunol. 2010 22(4):271-80(PMID: 20133493).
References by users
Comments
Deposited by Tsukamoto, H.
Restriction C: A prior written permission of the originator is necessary.
Photo