細胞株の詳細データ

TKG 0802:: CLC12N2

ID TKG 0802
Cell name CLC12N2
Data accepted January, 2020.
Animal Human.
Scientific name Homo sapiens.
Sex
Case history
Tissue Colon, adenocarcinoma.
Genetics
Life span Infinite.
Morphology
Medium RPMI1640 + 10% FBS.
Passage method 0.05% trypsin/EDTA.
Characteristics This cell line was established from COLO205 (TKG 0457) cell line.
(COLO205 cells were cotransfected with pEF-FLAG-C12N2 and a vector carrying the puromycin-resistance gene.)
Transplantable tumor cell line (subcutaneously, KSN nu/nu).
Temperature 37℃
CO2 concentration 5%
Subculture frequency 1:10 ~ 1:20, twice a week.
Classification
Mycoplasma PCR: -, Hoechst DNA staining: -
Established by Suda, T.
References Cancer Sci. 2010. 101:1822-1827 (PMID: 20500518).
References by users
Comments
Deposited by Suda, T.
Restriction In publishing the research results obtained by use of this cell line, a citation of the following literature designated by the originator is requested.
Cancer Sci. 2010. 101:1822-1827 (PMID: 20500518).
DNA Profile
(STR-PCR)
D5S818 D13S317 D7S820 D16S539 vWA TH01 Amelogenin TPOX CSF1PO
10,13 10,12 9,10 12,13 15 8,9 X 11 11,12
Photo