細胞株の詳細データ
TKG 0819：： MAY.035
| ID | TKG 0819 |
| Cell name | MAY.035 |
| Data accepted | March, 2025. |
| Animal | Mouse (BALB/c) hybridoma. |
| Scientific name | Mus musculus. |
| Sex | |
| Case history | |
| Tissue | Spleen cells x myeloma (Sp2/0). |
| Genetics | |
| Life span | Infinite. |
| Morphology | Lymphoblast-like. |
| Medium | RPMI1640 + 5% FBS |
| Passage method | Dilution. |
| Characteristics | The hybridoma secreting CD11a monoclonal antibody (IgG1). A BALB/c mouse was immunized with human EBV-transformed B-lymphoblastic cell line cells. A culture submitted to Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by treatments with BM Cyclin and MC210. |
| Temperature | 37℃ |
| CO2 concentration | 5% |
| Subculture frequency | 1:4 ~ 1:9, twice a week. |
| Classification | |
| Mycoplasma | PCR: -, Hoechst DNA staining: - |
| Established by | Ohashi Y. |
| References | Tohoku J Exp Med. 1992 Dec;168(4):599-610. (PMID: 1339124). |
| References by users | |
| Comments | |
| Deposited by | Ohashi Y. |
| Restriction | |
| Photo |  |