細胞株の詳細データ

TKG 0144 :: B16

ID: TKG 0144
Cell name: B16
Data accepted:
Animal: Mouse (C57BL/6)
Scientific name: Mus musculus
Sex:
Case history: sp.
Tissue: Skin, melanoma
Genetics: Virus detected, culturable
Life span: Infinite.
Morphology:
Medium: RPMI-1640 + 10% FBS.
Passage method: 0.05% trypsin
Characteristics: A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma.  Progeny were cured by Treatments with BM Cyclin and MC210.  This tissue culture cell line was established from a cell line maintained by in vivo transplantation. Conditions for transplantation, sc:90-100:20-40 (transplantation site : transplantation rate : survival days).
Temperature: 37℃
CO2 concentration: 5%
Subculture frequency: 1:20 ~ 1:40, twice a week.
Classification:
Mycoplasma: PCR: -, Hoechst DNA staining: -
Isozyme analysis: LD
Established by: Silagi, S.
References: Nagoya J. Med. Sci., 17, 102-110, 1954., An. N.Y. Acad. Sci., 100, 762-790, 1963., Cancer Res., 24, 1634-1643, 1964. (PMID: 14234007), J Cell Biol., 43, 263-274, 1969. (PMID: 4981070), FEBS Lett., 42, 32-35, 1974. (PMID: 4369215), Cancer Res., 38, 635-643, 1978. (PMID: 626968), J. Cell Physiol., 94, 265-273, 1978. (PMID: 202603), J. Cell Physiol., 116, 93-97, 1983. (PMID: 6406524)
References by users: Biol. Pharm. Bull., 23, 298-304, 2000. (PMID: 10726882), Oncology, 59, 75-80, 2000. (PMID: 10895071)
Deposited by:
Restriction:
Photo:     増殖後