細胞株の詳細データ
TKG 0144 ：： B16
| ID | TKG 0144 |
| Cell name | B16 |
| Data accepted | |
| Animal | Mouse (C57BL/6) |
| Scientific name | Mus musculus |
| Sex | |
| Case history | sp. |
| Tissue | Skin, melanoma |
| Genetics | Virus detected, culturable |
| Life span | Infinite. |
| Morphology | |
| Medium | RPMI-1640 + 10% FBS. |
| Passage method | 0.05% trypsin |
| Characteristics | A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210. This tissue culture cell line was established from a cell line maintained by in vivo transplantation. Conditions for transplantation, sc:90-100:20-40 (transplantation site : transplantation rate : survival days). |
| Temperature | 37℃ |
| CO2 concentration | 5% |
| Subculture frequency | 1:20 ~ 1:40, twice a week. |
| Classification | |
| Mycoplasma | PCR: -, Hoechst DNA staining: - |
| Isozyme analysis | LD |
| Established by | Silagi, S. |
| References | Nagoya J. Med. Sci., 17, 102-110, 1954., An. N.Y. Acad. Sci., 100, 762-790, 1963., Cancer Res., 24, 1634-1643, 1964. (PMID: 14234007), J Cell Biol., 43, 263-274, 1969. (PMID: 4981070), FEBS Lett., 42, 32-35, 1974. (PMID: 4369215), Cancer Res., 38, 635-643, 1978. (PMID: 626968), J. Cell Physiol., 94, 265-273, 1978. (PMID: 202603), J. Cell Physiol., 116, 93-97, 1983. (PMID: 6406524) |
| References by users | Biol. Pharm. Bull., 23, 298-304, 2000. (PMID: 10726882), Oncology, 59, 75-80, 2000. (PMID: 10895071), Oncol Rep. 23: 1425-1433, 2010.(PMID:20372860), Mol Cell Biol. 2019 Aug 12;39(17):e00154-19(PMID: 31208979). |
| Deposited by | |
| Restriction | |
| Photo |   増殖後 |