ID |
TKG 0154 |
Cell name |
L1210 |
Data accepted |
|
Animal |
Mouse (BDF1) |
Scientific name |
Mus musculus. |
Sex |
F. |
Case history |
MCA |
Tissue |
Spleen lymph node, leukemia. |
Genetics |
|
Life span |
Infinite. |
Morphology |
|
Medium |
RPMI-1640 + 10% Horse serum or RPMI-1640 + 10% FBS. |
Passage method |
Dilution. |
Characteristics |
This tissue culture cell line was established from a
cell line maintained by in vivo transplantation. Conditions for transplantation, ip:100:7-10 (transplantation site : transplantation
rate : survival days). Culture cell line was found to be contaminated with
mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210.
|
Temperature |
37℃ |
CO2 concentration |
5% |
Subculture frequency |
|
Classification |
|
Mycoplasma |
PCR: -, Hoechst DNA staining: - |
Isozyme analysis |
NP |
Established by |
|
References |
J. Natl. Cancer Inst., 10, 179-192, 1949(PMID: 18143260)., Cancer Res., 20, 930-939, 1960(PMID: 13823721)., Cancer Chemother. Rep., 51, 451-453, 1967. |
References by users |
Int. J. Rad. Appl. Instrum. B, 16, 371-374, 1989(PMID: 2777577)., Int. J. Rad. Appl. Instrum. B, 363-367, 1990(PMID: 2387744). |
Comments |
From NCI.
|
Deposited by |
|
Restriction |
|
Photo |
x100 |