細胞株の詳細データ
TKG 0173 :: S180 (Sarcoma 180)
ID | TKG 0173 |
Cell name | S180 (Sarcoma 180) |
Data accepted | |
Animal | Mouse (CFW, this cell line is transplanted in ddY mouse at present time). |
Scientific name | Mus musculus. |
Sex | |
Case history | |
Tissue | Sarcoma (Swiss Webster Sarcoma180). |
Genetics | |
Life span | Infinite. |
Morphology | |
Medium | RPMI-1640 + 10% FBS or D-MEM + 10% FBS. |
Passage method | Dilution. |
Characteristics | This tissue culture cell line was established from a cell line maintained by in vivo transplantation. Conditions for transplantation, ip:100:20 (transplantation site : transplantation rate : survival days). (transplanted in ddY mouse) cancer chemotherapy screening test. |
Temperature | 37℃ |
CO2 concentration | 5% |
Subculture frequency | 1:4~1:10, twice a week. |
Classification | |
Mycoplasma | PCR: -, Hoechst DNA staining: - |
Isozyme analysis | LD |
Established by | |
References | Cancer Res., 12, 422-425, 1952(PMID: 14936007)., J. Natl Cancer Inst., 13, 1299-1377, 1953(PMID: 13035452)., Proc. Soc. Exp. Biol. Med., 92, 347, 1956(PMID: 13350343)., Cancer Res., 20, 930-939, 1960(PMID: 13823721)., Imp. Cancer Res., Fund XIV, 122, 1977. |
References by users | S. T. P. Pharma Sciences, 8, 175-181, 1998. |
Comments | |
Deposited by | Dep. Internal Med. RITC. |
Restriction | |
Photo | Photo(x100) |