| ID |
TKG 0158 |
| Cell name |
Meth-A |
| Data accepted |
|
| Animal |
Mouse (BALB/c) |
| Scientific name |
Mus musculus. |
| Sex |
F. |
| Case history |
MC. |
| Tissue |
Fibrosarcoma, subcutaneous.
|
| Genetics |
|
| Life span |
Infinite. |
| Morphology |
|
| Medium |
RPMI-1640 + 10% FBS. |
| Passage method |
Dilution (1/5-1/10 every 2-3days) |
| Characteristics |
A culture submitted to the Cell Resource Center for Biomedical Research
was found to be contaminated with mycoplasma. Progeny were cured by Treatments
with BM Cyclin and MC210. Tissue culture cell line was established from
in
vivo transplantation cell line. Conditions for transplantation, ip:100:25-30/sc:100:12 (transplantation
site : transplantation rate : survival days). |
| Temperature |
37℃ |
| CO2 concentration |
5% |
| Subculture frequency |
|
| Classification |
|
| Mycoplasma |
PCR: -, Hoechst DNA staining: - |
| Isozyme analysis |
LD |
| Established by |
|
| References |
Proc. NAS., 83, 3121-3125, 1986. PMID: 3458168, Cancer Res., 41, 1301-1305, 1981. PMID: 6971146, Gann, 72, 692-699, 1981. PMID: 7327369, Radiol., 129, 225-227, 1978. PMID: 693882, Cancer Res., 38, 3006-3011, 1978. PMID: 679208, Cancer Res., 37, 3406-3413, 1977. PMID: 69494 |
| References by users |
|
| Comments |
From SLK laboratory. |
| Deposited by |
|
| Restriction |
|
| Photo |
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