| ID |
TKG 0168 |
| Cell name |
P815 |
| Data accepted |
|
| Animal： |
Mouse (DBA/2). |
| Scientific name |
Mus musculus. |
| Sex |
|
| Case history |
|
| Tissue |
Lympho node, mastocytoma.
|
| Genetics |
|
| Life span |
Infinite. |
| Morphology |
|
| Medium |
RPMI-1640 + 10% FBS. |
| Passage method |
0.05% trypsin or 0.25% trypsin. |
| Characteristics |
This tissue culture cell line was established from a
cell line maintained by in vivo transplantation. Conditions for transplantation, ip:100:7-10 (transplantation site : transplantation
rate : survival days). |
| Temperature |
37℃ |
| CO2 concentration |
5% |
| Subculture frequency |
1:10, twice a week. |
| Classification |
|
| Mycoplasma |
PCR: -, Hoechst DNA staining: - |
| Isozyme analysis |
LD |
| Established by |
Raiph, P. (SKI). |
| References |
Biochem. Biophys. Res. Commun., 61, 1268-1275, 1974. (PMID: 4616699), Cell Immunol., 9, 60-66, 1973.(PMID: 4270287), Nature, 249, 49-51, 1974.(PMID: 4208429), Cancer Res., 37, 546-550, 1977.(PMID: 318922), J. Immunol., 111, 389-394, 1973.(PMID: 4123976), J. Exp. Res., 143, 1528-1533, 1976.(PMID: 1083890) |
| References by users |
|
| Comments |
Obtained from Potter (NIH:1971).
|
| Deposited by |
|
| Restriction |
|
| Photo |
 
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