細胞株の詳細データ

TKG 0173 :: S180 (Sarcoma 180)

ID TKG 0173
Cell name S180 (Sarcoma 180)
Data accepted
Animal Mouse (CFW, this cell line is transplanted in ddY mouse at present time).
Scientific name Mus musculus.
Sex
Case history
Tissue Sarcoma (Swiss Webster Sarcoma180).
Genetics
Life span Infinite.
Morphology
Medium RPMI-1640 + 10% FBS or D-MEM + 10% FBS.
Passage method Dilution.
Characteristics This tissue culture cell line was established from a cell line maintained by in vivo transplantation. Conditions for transplantation, ip:100:20 (transplantation site : transplantation rate : survival days). (transplanted in ddY mouse) cancer chemotherapy screening test.
Temperature 37℃
CO2 concentration 5%
Subculture frequency 1:4~1:10, twice a week.
Classification
Mycoplasma PCR: -, Hoechst DNA staining: -
Isozyme analysis LD
Established by
References Cancer Res., 12, 422-425, 1952(PMID: 14936007)., J. Natl Cancer Inst., 13, 1299-1377, 1953(PMID: 13035452)., Proc. Soc. Exp. Biol. Med., 92, 347, 1956(PMID: 13350343)., Cancer Res., 20, 930-939, 1960(PMID: 13823721)., Imp. Cancer Res., Fund XIV, 122, 1977.
References by users S. T. P. Pharma Sciences, 8, 175-181, 1998.
Comments
Deposited by Dep. Internal Med. RITC.
Restriction
Photo
Photo(x100)